Splet23. avg. 2024 · The first step in a western blot is to generate a replica of the SDS-PAGE gel by transferring proteins electrophoretically to a synthetic membrane with a high protein binding capacity. In our experiments, we will use membranes made of polyvinylidine fluoride (PVDF), a kind of plastic. SpletWestern blot: technique, theory, and trouble shooting Western blot: technique, theory, and trouble shooting Authors Tahrin Mahmood 1 , Ping-Chang Yang Affiliation 1 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada. PMID: 23050259 PMCID: PMC3456489 DOI: 10.4103/1947-2714.100998

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